Type III ANOVA on main and interaction effects, and LM model summaries.
Prolactin
Chisq
Df
Pr(>Chisq)
(Intercept)
250.7924480
1
<0.0001
***
Study
3.2136720
1
0.07
ns
f39_gdm_new
0.5754347
1
0.4
ns
BMI
3.3368238
1
0.07
ns
Ethnicity_cauc
105.4219556
1
<0.0001
***
f10c_any_cig_1st_vst
49.1590682
1
<0.0001
***
GA_samp
164.6323095
1
<0.0001
***
Study:f39_gdm_new
1.8652579
1
0.2
ns
HPL
Chisq
Df
Pr(>Chisq)
(Intercept)
150.8759535
1
<0.0001
***
Study
7.3986291
1
0.007
**
f39_gdm_new
0.4357432
1
0.5
ns
BMI
236.2094019
1
<0.0001
***
Ethnicity_cauc
5.5165373
1
0.02
*
f10c_any_cig_1st_vst
0.9176455
1
0.3
ns
GA_samp
2601.9722143
1
<0.0001
***
Study:f39_gdm_new
1.4845286
1
0.2
ns
GH2
Chisq
Df
Pr(>Chisq)
(Intercept)
251.096669
1
<0.0001
***
Study
1.107622
1
0.3
ns
f39_gdm_new
1.386104
1
0.2
ns
BMI
87.622496
1
<0.0001
***
Ethnicity_cauc
3.246832
1
0.07
ns
f10c_any_cig_1st_vst
1.039153
1
0.3
ns
GA_samp
1032.266869
1
<0.0001
***
Study:f39_gdm_new
3.376951
1
0.07
ns
Insulin
model term
df1
df2
F.ratio
Chisq
p.value
1
Study
1
Inf
21.326
21.326
<0.0001
***
3
f39_gdm_new
1
Inf
33.726
33.726
<0.0001
***
4
BMI
1
Inf
330.722
330.722
<0.0001
***
5
Ethnicity_cauc
1
Inf
25.046
25.046
<0.0001
***
6
f10c_any_cig_1st_vst
1
Inf
3.158
3.158
0.08
ns
7
GA_samp
1
Inf
2.506
2.506
0.1
ns
2
Study:f39_gdm_new
1
Inf
4.089
4.089
0.04
*
HOMA.IR
model term
df1
df2
F.ratio
Chisq
p.value
1
Study
1
Inf
36.051
36.051
<0.0001
***
3
f39_gdm_new
1
Inf
32.027
32.027
<0.0001
***
4
BMI
1
Inf
316.807
316.807
<0.0001
***
5
Ethnicity_cauc
1
Inf
24.882
24.882
<0.0001
***
6
f10c_any_cig_1st_vst
1
Inf
2.618
2.618
0.1
ns
7
GA_samp
1
Inf
1.602
1.602
0.2
ns
2
Study:f39_gdm_new
1
Inf
4.361
4.361
0.04
*
GLUC3_mmol.L
model term
df1
df2
F.ratio
Chisq
p.value
1
Study
1
Inf
53.182
53.182
<0.0001
***
3
f39_gdm_new
1
Inf
4.345
4.345
0.04
*
4
BMI
1
Inf
11.449
11.449
0.0007
***
5
Ethnicity_cauc
1
Inf
0.718
0.718
0.4
ns
6
f10c_any_cig_1st_vst
1
Inf
0.028
0.028
0.9
ns
7
GA_samp
1
Inf
0.278
0.278
0.6
ns
2
Study:f39_gdm_new
1
Inf
1.558
1.558
0.2
ns
67.3.2 Model summary
Ratio of Geometric means (95% CI)
Prolactin
Var
Effect
P
(Intercept)
2.58 (2.21, 2.96)
<0.0001
B12
0.00 (-0.00, 0.00)
0.05
f39_gdm_newGDM
-0.14 (-0.42, 0.15)
0.35
StudySTOP
-0.02 (-0.20, 0.16)
0.84
GA_samp
0.14 (0.12, 0.16)
<0.0001
B12:f39_gdm_newGDM
0.00 (-0.00, 0.00)
0.67
f39_gdm_newGDM:StudySTOP
0.16 (-0.06, 0.39)
0.16
B12:StudySTOP
-0.00 (-0.00, 0.00)
0.87
HPL
Var
Effect
P
(Intercept)
0.57 (0.37, 0.76)
<0.0001
B12
0.00 (-0.00, 0.00)
0.08
f39_gdm_newGDM
-0.01 (-0.16, 0.13)
0.86
StudySTOP
0.09 (-0.01, 0.19)
0.07
GA_samp
0.29 (0.28, 0.30)
<0.0001
B12:f39_gdm_newGDM
-0.00 (-0.00, 0.00)
0.22
f39_gdm_newGDM:StudySTOP
0.08 (-0.04, 0.19)
0.18
B12:StudySTOP
0.00 (-0.00, 0.00)
0.96
GH2
Var
Effect
P
(Intercept)
-2.81 (-3.07, -2.54)
<0.0001
B12
0.00 (0.00, 0.00)
<0.0001
f39_gdm_newGDM
-0.18 (-0.39, 0.02)
0.09
StudySTOP
0.10 (-0.04, 0.23)
0.16
GA_samp
0.26 (0.25, 0.28)
<0.0001
B12:f39_gdm_newGDM
0.00 (-0.00, 0.00)
0.82
f39_gdm_newGDM:StudySTOP
0.19 (0.02, 0.35)
0.03
B12:StudySTOP
-0.00 (-0.00, -0.00)
0.02
Insulin
Var
Effect
P
(Intercept)
2.56 (2.07, 3.06)
<0.0001
B12
-0.00 (-0.00, 0.00)
0.13
f39_gdm_newGDM
1.24 (0.32, 2.17)
0.009
StudySTOP
-0.30 (-0.56, -0.03)
0.03
GA_samp
0.02 (-0.01, 0.05)
0.24
B12:f39_gdm_newGDM
-0.00 (-0.00, 0.00)
0.25
f39_gdm_newGDM:StudySTOP
-0.61 (-1.61, 0.39)
0.23
B12:StudySTOP
0.00 (-0.00, 0.00)
0.60
B12:f39_gdm_newGDM:StudySTOP
0.00 (-0.00, 0.00)
0.42
HOMA.IR
Var
Effect
P
(Intercept)
1.19 (0.67, 1.71)
<0.0001
B12
-0.00 (-0.00, 0.00)
0.16
f39_gdm_newGDM
1.28 (0.24, 2.32)
0.02
StudySTOP
-0.45 (-0.73, -0.16)
0.002
GA_samp
0.01 (-0.02, 0.04)
0.38
B12:f39_gdm_newGDM
-0.00 (-0.00, 0.00)
0.36
f39_gdm_newGDM:StudySTOP
-0.60 (-1.71, 0.51)
0.29
B12:StudySTOP
0.00 (-0.00, 0.00)
0.63
B12:f39_gdm_newGDM:StudySTOP
0.00 (-0.00, 0.00)
0.55
GLUC3_mmol.L
Var
Effect
P
(Intercept)
1.74 (1.65, 1.84)
<0.0001
B12
-0.00 (-0.00, 0.00)
0.16
f39_gdm_newGDM
0.11 (-0.04, 0.26)
0.15
StudySTOP
-0.15 (-0.20, -0.11)
<0.0001
GA_samp
-0.00 (-0.01, 0.00)
0.46
B12:f39_gdm_newGDM
0.00 (-0.00, 0.00)
0.85
f39_gdm_newGDM:StudySTOP
-0.08 (-0.20, 0.03)
0.16
B12:StudySTOP
0.00 (-0.00, 0.00)
0.55
67.3.3 Estimated means
Estimated marginal means (or adjusted means) adjusted for GA at sampling.
Results are averaged over the levels of B12 concentration (pM) and may be misleading when interaction term is involved.
Prolactin
Study
f39_gdm_new
response
SE
df
lower.CL
upper.CL
SCOPE
non-GDM
99.48282
7.527503
90.84823
85.59952
115.6178
STOP
non-GDM
96.25826
6.870852
72.18811
83.49184
110.9767
SCOPE
GDM
91.16056
11.371799
545.10747
71.34899
116.4732
STOP
GDM
103.59983
8.934423
149.87271
87.36834
122.8469
HPL
Study
f39_gdm_new
response
SE
df
lower.CL
upper.CL
SCOPE
non-GDM
90.26660
2.468158
171.5369
85.52386
95.27234
STOP
non-GDM
99.14269
2.415073
110.2077
94.47037
104.04610
SCOPE
GDM
82.56036
4.512539
818.3553
74.16144
91.91047
STOP
GDM
97.86903
3.519895
414.0479
91.18886
105.03858
GH2
Study
f39_gdm_new
response
SE
df
lower.CL
upper.CL
SCOPE
non-GDM
2.436758
0.0725078
439.7072
2.298340
2.583512
STOP
non-GDM
2.314613
0.0556715
242.8413
2.207509
2.426912
SCOPE
GDM
2.072222
0.1583053
1395.6170
1.783829
2.407240
STOP
GDM
2.371184
0.1004585
760.9642
2.181953
2.576826
Insulin
Study
f39_gdm_new
response
SE
df
asymp.LCL
asymp.UCL
SCOPE
non-GDM
13.77965
0.7143520
Inf
12.44832
15.25335
STOP
non-GDM
10.98705
0.4168576
Inf
10.19966
11.83522
SCOPE
GDM
27.81263
3.7909330
Inf
21.29222
36.32980
STOP
GDM
18.16724
1.3699737
Inf
15.67114
21.06091
HOMA.IR
Study
f39_gdm_new
response
SE
df
asymp.LCL
asymp.UCL
SCOPE
non-GDM
3.336606
0.1841394
Inf
2.994533
3.717755
STOP
non-GDM
2.287398
0.0913192
Inf
2.115239
2.473569
SCOPE
GDM
7.442549
1.1042775
Inf
5.564496
9.954455
STOP
GDM
3.907561
0.3165887
Inf
3.333817
4.580044
GLUC3_mmol.L
Study
f39_gdm_new
response
SE
df
asymp.LCL
asymp.UCL
SCOPE
non-GDM
5.402700
0.0546200
Inf
5.296700
5.510821
STOP
non-GDM
4.703314
0.0358930
Inf
4.633488
4.774191
SCOPE
GDM
6.070570
0.3237354
Inf
5.468094
6.739426
STOP
GDM
4.866900
0.0625328
Inf
4.745869
4.991019
67.3.4 Comparisons
67.3.4.1 Within Groups
Effect for every 100 unit increase in B12 concentration (pM).
Hormone
Contrast
Study
f39_gdm_new
Ratio of Geometric means (95% CI)
P-value
Prolactin
B12366 / B12266
SCOPE
non-GDM
1.044 (0.999, 1.092)
0.05
Prolactin
B12366 / B12266
STOP
non-GDM
1.040 (1.007, 1.074)
0.02
Prolactin
B12366 / B12266
SCOPE
GDM
1.061 (0.978, 1.150)
0.2
Prolactin
B12366 / B12266
STOP
GDM
1.056 (0.989, 1.128)
0.1
HPL
B12366 / B12266
SCOPE
non-GDM
1.022 (0.998, 1.046)
0.08
HPL
B12366 / B12266
STOP
non-GDM
1.022 (1.005, 1.041)
0.01
HPL
B12366 / B12266
SCOPE
GDM
0.997 (0.955, 1.041)
0.9
HPL
B12366 / B12266
STOP
GDM
0.998 (0.963, 1.034)
0.9
GH2
B12366 / B12266
SCOPE
non-GDM
1.073 (1.038, 1.109)
<0.0001
GH2
B12366 / B12266
STOP
non-GDM
1.024 (1.000, 1.049)
0.05
GH2
B12366 / B12266
SCOPE
GDM
1.080 (1.016, 1.147)
0.01
GH2
B12366 / B12266
STOP
GDM
1.031 (0.981, 1.083)
0.2
Insulin
B12366 / B12266
SCOPE
non-GDM
0.948 (0.885, 1.016)
0.1
Insulin
B12366 / B12266
STOP
non-GDM
0.969 (0.925, 1.016)
0.2
Insulin
B12366 / B12266
SCOPE
GDM
0.799 (0.600, 1.064)
0.1
Insulin
B12366 / B12266
STOP
GDM
0.929 (0.841, 1.026)
0.1
HOMA.IR
B12366 / B12266
SCOPE
non-GDM
0.948 (0.880, 1.021)
0.2
HOMA.IR
B12366 / B12266
STOP
non-GDM
0.969 (0.922, 1.018)
0.2
HOMA.IR
B12366 / B12266
SCOPE
GDM
0.815 (0.594, 1.117)
0.2
HOMA.IR
B12366 / B12266
STOP
GDM
0.925 (0.831, 1.029)
0.1
GLUC3_mmol.L
B12366 / B12266
SCOPE
non-GDM
0.991 (0.979, 1.004)
0.2
GLUC3_mmol.L
B12366 / B12266
STOP
non-GDM
0.996 (0.988, 1.003)
0.3
GLUC3_mmol.L
B12366 / B12266
SCOPE
GDM
0.994 (0.964, 1.024)
0.7
GLUC3_mmol.L
B12366 / B12266
STOP
GDM
0.998 (0.977, 1.020)
0.9
67.3.4.2 Between Groups
Below are comparisons of the differences in the change in B12 concentration (pM) between groups. I.e. whether the change in B12 concentration (pM) are different between groups.
Prolactin
contrast
ratio
SE
df
lower.CL
upper.CL
p.value
SCOPE/SCOPE (Overall)
0.9913717
0.0545354
2067.758
0.8899886
1.104304
0.875
ns
STOP/SCOPE (non-GDM)
0.9956765
0.0273861
2067.758
0.9433921
1.050859
0.875
ns
STOP/SCOPE (GDM)
0.9956765
0.0273861
2067.758
0.9433921
1.050859
0.875
ns
GDM/non-GDM (SCOPE)
1.0156870
0.0371835
2042.467
0.9453216
1.091290
0.671
ns
GDM/non-GDM (STOP)
1.0156870
0.0371835
2042.467
0.9453216
1.091290
0.671
ns
HPL
contrast
ratio
SE
df
lower.CL
upper.CL
p.value
SCOPE/SCOPE (Overall)
1.0014580
0.0295309
1931.787
0.9451851
1.061081
0.961
ns
STOP/SCOPE (non-GDM)
1.0007287
0.0147547
1931.787
0.9722063
1.030088
0.961
ns
STOP/SCOPE (GDM)
1.0007287
0.0147547
1931.787
0.9722063
1.030088
0.961
ns
GDM/non-GDM (SCOPE)
0.9763306
0.0192592
1973.738
0.9392813
1.014841
0.225
ns
GDM/non-GDM (STOP)
0.9763306
0.0192592
1973.738
0.9392813
1.014841
0.225
ns
GH2
contrast
ratio
SE
df
lower.CL
upper.CL
p.value
SCOPE/SCOPE (Overall)
0.9111097
0.0372634
2095.061
0.8408864
0.9871974
0.023
*
STOP/SCOPE (non-GDM)
0.9545206
0.0195194
2095.061
0.9169986
0.9935781
0.023
*
STOP/SCOPE (GDM)
0.9545206
0.0195194
2095.061
0.9169986
0.9935781
0.023
*
GDM/non-GDM (SCOPE)
1.0064190
0.0275758
2103.809
0.9537676
1.0619770
0.815
ns
GDM/non-GDM (STOP)
1.0064190
0.0275758
2103.809
0.9537676
1.0619770
0.815
ns
Insulin
contrast
ratio
SE
df
asymp.LCL
asymp.UCL
p.value
SCOPE/SCOPE (Overall)
1.1891756
0.1906466
Inf
0.8685259
1.628205
0.280
ns
STOP/SCOPE (non-GDM)
1.0222629
0.0433951
Inf
0.9406521
1.110954
0.604
ns
STOP/SCOPE (GDM)
1.1632777
0.1798378
Inf
0.8591940
1.574982
0.328
ns
GDM/non-GDM (SCOPE)
0.8423908
0.1265802
Inf
0.6274940
1.130883
0.254
ns
GDM/non-GDM (STOP)
0.9585934
0.0535768
Inf
0.8591319
1.069570
0.449
ns
HOMA.IR
contrast
ratio
SE
df
asymp.LCL
asymp.UCL
p.value
SCOPE/SCOPE (Overall)
1.1596192
0.2039332
Inf
0.8215258
1.636853
0.400
ns
STOP/SCOPE (non-GDM)
1.0217772
0.0463690
Inf
0.9348201
1.116823
0.635
ns
STOP/SCOPE (GDM)
1.1349042
0.1928267
Inf
0.8134581
1.583373
0.456
ns
GDM/non-GDM (SCOPE)
0.8593715
0.1420847
Inf
0.6215084
1.188269
0.359
ns
GDM/non-GDM (STOP)
0.9545175
0.0572632
Inf
0.8486308
1.073616
0.438
ns
GLUC3_mmol.L
contrast
ratio
SE
df
asymp.LCL
asymp.UCL
p.value
SCOPE/SCOPE (Overall)
1.009181
0.0155431
Inf
0.9791723
1.040109
0.553
ns
STOP/SCOPE (non-GDM)
1.004580
0.0077361
Inf
0.9895313
1.019858
0.553
ns
STOP/SCOPE (GDM)
1.004580
0.0077361
Inf
0.9895313
1.019858
0.553
ns
GDM/non-GDM (SCOPE)
1.002406
0.0127711
Inf
0.9776852
1.027752
0.850
ns
GDM/non-GDM (STOP)
1.002406
0.0127711
Inf
0.9776852
1.027752
0.850
ns
67.3.5 Estimated means (50 unit increase)
Estimated marginal means (or adjusted means) adjusted for GA at sampling.
Results are averaged over the levels of B12 concentration (pM) and may be misleading when interaction term is involved.
Prolactin
Study
f39_gdm_new
response
SE
df
lower.CL
upper.CL
SCOPE
non-GDM
98.40719
7.379890
87.68820
84.78112
114.2233
STOP
non-GDM
95.32069
6.798638
71.96751
82.68715
109.8845
SCOPE
GDM
89.82470
11.103764
530.19810
70.45843
114.5140
STOP
GDM
102.19232
8.780994
147.75222
86.23305
121.1052
HPL
Study
f39_gdm_new
response
SE
df
lower.CL
upper.CL
SCOPE
non-GDM
89.78496
2.407079
159.1047
85.15468
94.66702
STOP
non-GDM
98.59574
2.398004
109.5640
93.95596
103.46463
SCOPE
GDM
82.61309
4.461095
808.5369
74.30451
91.85071
STOP
GDM
97.91371
3.500391
404.8910
91.26874
105.04249
GH2
Study
f39_gdm_new
response
SE
df
lower.CL
upper.CL
SCOPE
non-GDM
2.394278
0.0688602
393.2630
2.262654
2.533559
STOP
non-GDM
2.300881
0.0551683
240.6173
2.194733
2.412163
SCOPE
GDM
2.032842
0.1535026
1385.4263
1.752961
2.357411
STOP
GDM
2.353349
0.0987469
746.1677
2.167264
2.555412
Insulin
Study
f39_gdm_new
response
SE
df
asymp.LCL
asymp.UCL
SCOPE
non-GDM
13.96429
0.6920242
Inf
12.67174
15.38869
STOP
non-GDM
11.07315
0.4138377
Inf
10.29104
11.91470
SCOPE
GDM
29.42012
4.0226695
Inf
22.50391
38.46191
STOP
GDM
18.50421
1.3916351
Inf
15.96816
21.44304
HOMA.IR
Study
f39_gdm_new
response
SE
df
asymp.LCL
asymp.UCL
SCOPE
non-GDM
3.381458
0.1782188
Inf
3.049592
3.749440
STOP
non-GDM
2.305694
0.0907315
Inf
2.134549
2.490562
SCOPE
GDM
7.833855
1.1876586
Inf
5.820072
10.544420
STOP
GDM
3.984922
0.3226905
Inf
3.400097
4.670338
GLUC3_mmol.L
Study
f39_gdm_new
response
SE
df
asymp.LCL
asymp.UCL
SCOPE
non-GDM
5.414391
0.0518294
Inf
5.313754
5.516934
STOP
non-GDM
4.708110
0.0358237
Inf
4.638418
4.778849
SCOPE
GDM
6.080052
0.3296930
Inf
5.467019
6.761827
STOP
GDM
4.868938
0.0624862
Inf
4.747994
4.992961
67.3.6 Comparisons (50 unit increase)
67.3.6.1 Within Groups
Effect for every 50 unit increase in B12 concentration (pM).
Hormone
Contrast
Study
f39_gdm_new
Ratio of Geometric means (95% CI)
P-value
Prolactin
B12316 / B12266
SCOPE
non-GDM
1.022 (1.000, 1.045)
0.05
Prolactin
B12316 / B12266
STOP
non-GDM
1.020 (1.003, 1.036)
0.02
Prolactin
B12316 / B12266
SCOPE
GDM
1.030 (0.989, 1.073)
0.2
Prolactin
B12316 / B12266
STOP
GDM
1.028 (0.994, 1.062)
0.1
HPL
B12316 / B12266
SCOPE
non-GDM
1.011 (0.999, 1.023)
0.08
HPL
B12316 / B12266
STOP
non-GDM
1.011 (1.002, 1.020)
0.01
HPL
B12316 / B12266
SCOPE
GDM
0.999 (0.977, 1.020)
0.9
HPL
B12316 / B12266
STOP
GDM
0.999 (0.981, 1.017)
0.9
GH2
B12316 / B12266
SCOPE
non-GDM
1.036 (1.019, 1.053)
<0.0001
GH2
B12316 / B12266
STOP
non-GDM
1.012 (1.000, 1.024)
0.05
GH2
B12316 / B12266
SCOPE
GDM
1.039 (1.008, 1.071)
0.01
GH2
B12316 / B12266
STOP
GDM
1.015 (0.990, 1.041)
0.2
Insulin
B12316 / B12266
SCOPE
non-GDM
0.974 (0.941, 1.008)
0.1
Insulin
B12316 / B12266
STOP
non-GDM
0.985 (0.962, 1.008)
0.2
Insulin
B12316 / B12266
SCOPE
GDM
0.894 (0.774, 1.031)
0.1
Insulin
B12316 / B12266
STOP
GDM
0.964 (0.917, 1.013)
0.1
HOMA.IR
B12316 / B12266
SCOPE
non-GDM
0.974 (0.938, 1.010)
0.2
HOMA.IR
B12316 / B12266
STOP
non-GDM
0.984 (0.960, 1.009)
0.2
HOMA.IR
B12316 / B12266
SCOPE
GDM
0.903 (0.771, 1.057)
0.2
HOMA.IR
B12316 / B12266
STOP
GDM
0.962 (0.912, 1.014)
0.1
GLUC3_mmol.L
B12316 / B12266
SCOPE
non-GDM
0.996 (0.990, 1.002)
0.2
GLUC3_mmol.L
B12316 / B12266
STOP
non-GDM
0.998 (0.994, 1.002)
0.3
GLUC3_mmol.L
B12316 / B12266
SCOPE
GDM
0.997 (0.982, 1.012)
0.7
GLUC3_mmol.L
B12316 / B12266
STOP
GDM
0.999 (0.988, 1.010)
0.9
67.3.6.2 Between Groups
Below are comparisons of the differences in the change in B12 concentration (pM) between groups. I.e. whether the change in B12 concentration (pM) are different between groups.